1.    Range for 16OHE1 in urine of healthy women (first morning collection):

2 - 40 ng/ml (premenopausal)

0.6 - 10 ng/ml (postmenopausal)

2.    Range for 2OHE1 in urine of healthy women:

4 - 100 ng/ml (premenopausal)

1 - 20 ng/ml (postmenopausal)

3.    Range for Estrogen Metabolite Index (2OHE1/16OHE1):

0.6 - 6 (in "healthy" pre- and postmenopausal women)

4.    Mean Value for Estrogen Metabolite Index for healthy women:

2.3 (premenopausal)

2.0 (postmenopausal)

Standard Curves

Below are typical standard curves for EIA of urinary 2OHE1 and 16OHE1. We highly recommend use of a four-parameter curve to fit the standards.

Standard Curves for EIA of urinary 2-Hydroxyestrogen and 16a-hydroxyestrone;  nanograms/ml versus rate of change of optical density at 405 nm (milli O.D. units/minute).